Figure 2: Mutation of the SMR motif results in delayed viral release and decreased infectivity. (A) 5.0 X105 A3.01 T cells were infected with HIVwt, HIVdsNef, or HIVNefsmr5a virus and assayed by ELISA for changes in p24 release in culture medium at 3-day intervals. Differences in viral replication kinetics were observed between the various viral clones. (B) A3.01-derived viral preparations were assessed for infectivity by MAGI assay. HIVNefsmr5a and HIVdsNef viral particles were found to be less infectious than HIVwt viral particles by 10-fold and 100-fold respectively. Plots shown are a representation of three independent experiments.