Specificity Clone Isotype Manufacturer Fluorochrome
HLA-DR L234 IgG2a BioLegend PB
CD45 HI30 IgG1 Invitrogen PO
CD294 (CRTH2) BM16 IgG2a BioLegend PE
CD123 (IL-3R) 6H6 IgG1 BioLegend PE-Cy7
CD3 SK7 IgG1 BDB APC
Abbreviations: APC: Allophycocyanin; PE: Phycoerythrin; PB: Pacific Blue; PO: Pacific Orange; IL-3R: Interleukin 3 Receptor; CRTH2: G protein-coupled receptor-44 or chemoattractant receptor expressed on Th2 cells. Manufacturer: BioLegend®, San Diego, CA; Invitrogen®, Camarillo, CA; Becton Dickinson Biosciences (BDB), San Jose, CA
Table 1: Clones and specificities of the monoclonal antibodies used in this study.
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