Figure 2: Effects of n-alkanols on GDP/GTPγS exchange from Gαo subunit: Gαo subunit was preloaded with unlabeled GDP as described in Materials and Methods. The exchange of GTPγS for GDP was determined by trapping the protein on nitrocellulose filters and counting the radioactivity bound to protein. Values are % of control (Gαo subunit incubated without alcohol). Each value is mean + SE of at least three experiments assayed in duplicate. * Significant at p<0.05.