Figure 3: Effects of n-alkanols on GDP/GTPγS exchange from Gαi- 2subunit: Gαi2 subunit was preloaded with unlabeled GDP as described in Materials and Methods. The exchange of GTPγS for GDP was determined by trapping the protein on nitrocellulose filters and counting the radioactivity bound to protein. Values are % of control (Gαi2 subunit incubated without alcohol). Each value is mean + SE of at least three experiments assayed in duplicate. * Significant at p<0.05.