Gene Symbol SNP Allele1/ positionα/ Allele2 Type/change  MAF (%)† PCR primers Size (bp) Diagnostic fragments (bp) Enzyme Design ATP-binding cassette, sub-family B, member 1 ABCB1 rs1045642 C3435T Synon.   C 43 F AGCTGCTTGATGGCAAAGAAAT R TGGTCGAACACTTTCATCCCTT 433 T 387 C 236 MboI ‡   rs1128503 T1236C Synon.   T 44 F TCTTTGTCACTTTATCCAGC R TCTCACCATCCCCTCTGT 502 C 361 T 416 HaeIII § Catechol-O-methyltransferase COMT rs4680 Val158Met Non-synon.   A 39 F GTGGACGCCGTGATTCAGGAG R CAGGCATGCACACCTTGTCCgTC 199 A 107 G 83 Tsp45I ‡ Cytochrome P450, family 3, subfamily A, polypeptide 4 CYP3A4 rs2242480 C20230T Intronic   T 33 F ACCCTGATGTCCAGCAGAAACT R ATAGAAAGCAGATGAACCAGAGCC 284 C 216 T 284 RsaI ¶ Cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 rs776746 A6986G Splice-3 A  3 F GCATAGGAGATACCCACGTATcT R TGGTCCAAACAGGGAAGAGgTA 118 G 97 A 118 RsaI ** Neurobeachin1 NBEA rs7990537 A11771G Intronic G 28 F CCCTTGGATGTTTGAACCTCTG R TTTCCTAGATGCCCTTCACTGG 433 A 433 G 332 Bts1 ‡ Opioid Receptor, mu 1 OPRM1 rs1799971  A118G Non-synon.   G 19 F TCAACTTGTCCCACTTAGATCGC R TGACCAGGAAGTTTCCGAAGAG 180 A 180 G 158 BstUI ‡     rs2075572 IVS2-691 Intronic   G 40 F TAGCTCTGGTCAAGGCTAAAgAT R CCCAGTACCAGGTTGGATGAGA 154 G 154 C 134 MboI ‡ Potassium inwardly-rectifying channel, subfamily J, member 6 KCNJ6 rs2070995 A1032G Synon.   A 20 F TAGAGGACCCCTCCTGGACT R CGGAACATCAGGCACAGTTT 298 A 197 G 175 MspI ‡   rs6517442 G-1250A Upstream   G 24 F TGGCTATTCTTGTGCTGCTTT R CAGTCATTTTTAGAGGGCAGtCA 203 G 203 A 181 HincII ‡ Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 1 KCNS1 rs734784 Ile489Val Non-synon. G 40 F AGTTTGAGGACTTGCTGAGCcGC R ATACATCTGAGGGTGTGGAGGC 137 A 137 G 115 BstUI ‡ Sodium channel, voltage-gated, type IX, alpha subunit SCN9a rs6746030 Arg1150Trp Non-synon. A 11 F GTTTTCCTGATGTTCCACCAGATT R TGAAGGAAGGCAGCATCCAAATTA 244 A 244 G 172 RsaI ‡ Lower case in primer sequences indicates bases changed to alter restriction sites. Key: Type codes with protein level consequence: Synon.-synonomous,- no amino acid change; Non-synon.- non-synonymous, amino acid change as indicated; Intronic – variant occurs in a non-coding portion of the gene; upstream – in region upstream (5') of the gene; Slice-3 – causes transcript splice variation in 3′ of the gene; IVS2 –within intron 2 of the gene (full SNP code:IVS2-691 G>C); UTR-3′ - 3′ untranslated region. † Minor allele frequencies (MAF) are from http://www.ncbi.nlm.nih.gov/projects/SNP/. αChromosome positions are from HumRef sequence and transcript positions are from Genbank NM reference sequences. Primers and test design ‡ (us); §[44], ¶[45], **[46].

Table 1: SNPs examined in this study: allele frequency, PCR primer sequences and RFLP test details.