Figure 2: Effects of drug holiday schedule for prieurianin in DIO mice. A, B, Drug holiday treatment protocols for prieurianin in DIO mice. This on-off or cyclical treatment strategy comprised of specifi ed doses of prieurianin at either 3 or 5 mg kg-1 were given as indicated for a defi ned duration coupled with intermittent drug holiday to overcome drug-induced tolerance. C, D, Drug holiday treatment schedule with either 3 or 5 mg kg-1 prieurianin circumvented drug-induced tolerance and produced a sustained weight loss (C) and reduced food intake (D). All studies consisted of 10 animals per group, except for the 3 and 5 mg kg-1 prieurianin treated groups (n=20).