Figure 2: Effect of EA on GLUT4 translocation. 3T3-L1 preadipocytes stably expressing myc-GLUT4-GFP chimera were serum starved for 2 h and incubated with indicated concentrations of EA or insulin for 30 min. Myc epitope externalization was measured by indirect immunofluorescence. (A) Images showing cell surface GLUT4 and (B) showing fold increase in fluorescence intensity of secondary antibody upon treatment with insulin or EA compared to the basal. Cells treated with vehicle (0.1% DMSO) were used to measure non-specific fluorescence. Values are shown as the mean ± S.D. of three different focal planes of the same experiment. *P<0.05 vs. unstimulated cells.