Figure 4: Effect of EA on phosphorylation status of AS160, ERK 1/2 and aPKCζ/λ. Differentiated adipocytes and myotubes were treated with 50 nM insulin or 100 nM EA for 30 min. EA neither stimulated AS160 phosphorylation in adipocytes (A) nor in muscles (B). EA induced ERK and aPKC activation in both adipocytes (C & E) and muscles (D & F). Data shown are representative immunoblots of 3-5 independent experiments. Bars represent mean ± S.D. *P<0.05 compared with basal.