Figure 5: Effect of inhibitors on EA stimulated glucose transport. C2C12 myotubes were serum starved for 2 h, treated with or without 50 μM Compound C (CC) for 1 hour or 50 μM PD98059 (PD) for 30 min or 100 nM wortmannin (W) for 20 minutes. After inhibitor treatment, cells were stimulated with 100 nM EA in the presence of either PD or CC. Cells were also stimulated with EA in the absence of inhibitors. Cells incubated with vehicle (0.1% DMSO) were used to measure the basal rate of glucose uptake. Values are shown as the mean ± S.D. of three different experiments carried out in duplicates. *P<0.05 vs. unstimulated cells. #P<0.05 vs. EA stimulated cells.