Figure 3: TNF-α suppresses the secretion of adiponectin from 3T3-L1 adipocytes. 3T3-L1 adipocytes were transduced with Ad-resistin or AdlacZ. At 48 hrs after infection, 3T3-L1 adipocytes were serum-starved for 3 hrs prior to treatment without or with TNF-α (10 ng/mL) for 3 h. Conditioned medium was then collected, and fractionated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis, and immunoblotted using a polyclonal antibody against mouse adiponectin or mouse resistin determining the levels of secreted adiponectin or resistin. Relative levels of adiponectin released from adipocytes treated with TNF-α, as assessed from 4 independent experiments. **P < 0.01.