Figure 2: Cerebellar insulin and IGF-1 resistance following chronic prenatal ethanol exposure. P30 cerebella were used to measure Immunoreactivity to the (A) insulin receptor (IR), (B) IGF-1R, (C) IRS-1, (D) pYpY1162/1163-IR, (E) pYpY1135/1136-IGF-1R, (F) pS312-IRS-1 by multiplex ELISA. Phospho-/total protein ratios for (G) IR, (H) IGF-1R, and (I) IRS-1 were calculated. Inter-group comparisons were made using Student t-tests. Significant differences are indicated within the panels. N=12/group with each sample contributed from a different litter.