Figure 4: Effects of chronic prenatal ethanol exposure on Wnt signaling in juvenile cerebella. Probe-based qPCR amplification reactions were used to measure (A) Wnt 5a, (B) Wnt 5b, (C) Fzd 4, (D) Fzd 6, (E) Didxc 1A, (F) EP300, and (G) Axin 2. Results were normalized to β-actin, which was measured simultaneously in the duplex qPCR reactions. Inter-group comparisons were made with calculated mRNA/β-actin ratios. N=12/group with each sample contributed from a different litter.