Immunoreactivity Control Ethanol t-Test
Notch 1 20002.8 ± 1182.0 19892.7 ± 1028.2  
Jagged 1 12360.8 ± 590.9 9897.8 ± 1031.3 0.035
HES-1 3883.5 ± 111.2 3917.7 ± 159.2  
Humbug 18863.4 ± 1007.0 13628.9 ± 1318.8 0.003
AAH 10340.2 ± 491.6 7581.0 ± 710.3 0.003
β-Catenin 4824.5 ± 138.1 3828.6 ± 227.2 <0.001
Immunoreactivity was measured in P30 cerebellar tissue by duplex ELISA with results normalized to large ribosomal protein measured in the same wells. Results correspond to mean net fluorescence light units (± S.E.M.) in 50 ng protein. Data were generated from 12 rats per group, with each sample contributed from a different litter. Inter-group comparisons were made with Student T-tests
Table 1: Effects of Chronic Prenatal Ethanol Exposure on Notch and Wnt Pathway Protein Expression in Cerebellum.