Tissue Pathology No treatment Treatment with Vit D3
Lung Alveolar macrophages 1 0
  Inflammatory foci 2 1
Liver Inflammatory foci 3 2
  Hemorrhage 2 0
  Mitosis 1 0
Small intestine Inflammatory infiltrates 1 0
  Necrotic mucosal cells 2 1
Brain Presence of the parasite 2 0
Spleen Granulocytes 2 1
Histopathologic examination of the tissues was performed 7 days post inoculation. Numbers are based on severity of the lesions (0, no lesion, 1, mild, 2, slight, 3, moderate changes) and the total was divided the number of animals in the group. Also, in vitro studies with incubated intestinal epithelial cells showed a significant dose-dependent inhibition of intracellular T. gondii tachyzoites (RH strain, type I) proliferation at 10-7 mol/l of 1,25(OH)2D3 concentration
Table 41: Effect of pretreatment with 1,25(OH)2D3 (0.5 μg/kg/2 days) on tissue pathology caused by T. gondii avirulent ME49 strain infection with 20 cysts administered intraperitoneally in BALB/c mice (according to Rajapakse et al. [638]; with own modification).