Cohort 1 Cohort 2 Cohort 3
Polymorphisms Healthy Controls (n=200) DM(356) DNP (139) OR (95%CI) P DM (342) DNP (133) OR (95%CI) P DM (207) DNP (81) OR (95%CI) P
T-786C
(rs 2070744)
TT=131 (0.66)
TC=67 (0.33)
CC=2 (0.01) TC+CC=69 (0.34)
T=329 (0.82)
C=71 (0.18)
TT=199 (0.56)
TC=121 (0.34)
CC=36 (0.10) TC+CC=157 (0.44)
T=519 (0.73)
C=193 (0.27)
TT=72 (0.52)
TC=50 (0.36)
CC=17 (0.12) TC+CC=67 (0.48)\
T=194 (0.70)
C=84 (0.30)

1.22 (0.76-1.89) 0.79

1.30 (0.65-2.90) 0.72

1.11 (0.70-1.90) 0.61

1.16 (0.86-1.58) 0.37
TT=171 (0.50)
TC=130 (0.38)
CC=41 (0.12) TC+CC=166 (0.50) T=472 (0.69)
C=212 (0.31)
TT=66
(0.50)
TC=52 (0.39)
CC=15 (0.11) TC+CC=67 (0.50) T=184 (0.69)
C=82 (0.31)
1.12 (0.80-1.55) 0.98

1.15 (0.66-1.90) 1.02

1.12 (0.81-1.55) 0.80

0.99 (0.73-1.35) 1
TT=107 (0.52)
TC=75 (0.36)
CC=25 (0.12)
TC+CC=100 (0.48)
T=289 (0.70)
C=125 (0.30)
TT=40 (0.49)
TC=31 (0.39)
CC=10 (0.12)
TC+CC=41 (0.51)
T=111 (0.69)
C=51 (0.31)

1.22 ( 0.85-1.45) 0.90

1.11 (0.62-1.98) 0.94

1.20 (0.83-1.61) 1.02

1.06 (0.72-1.57) 0.84
G894T
(rs 1799983)
GG=102 (0.51) GT=80 (0.40) TT=18 (0.09) GT+TT=98 (0.49) G=284 (0.71)
T=116 (0.29)
GG=156 (0.44)
GT=164 (0.46)
TT=36 (0.10) GT+TT=200 (0.56) G=476 (0.67)
T=236 (0.33)
GG=65 (0.47)
GT=60 (0.43) TT=14 (0.10) GT+TT=74 (0.53) G=190 (0.68)
T=88 (0.32)
0.94 (0.59-1.60) 0.79 0.94 (0.42-2..22) 0.89 0.95 (0.59-1.52) 0.78 0.93 (0.69-1.26) 0.71 GG=157 (0.46)
GT=144 (0.42) TT=41 (0.12) GT+TT=185 (0.54) G=458 (0.67)
T=226 (0.33)
GG=63 (0.47)
GT=57 (0.43) TT=13 (0.10) GT+TT=70 (0.53) G=183 (0.69)
T=83 (0.31)
1.08 (0.72-1.52) 1.05 0.80 (0.41-1.52) 0.62 1.20 (0.77-1..48) 0.95 0.92 (0.68-1.25) 0.64 GG=97 (0.47)
GT=87 (0.42) TT=23 (0.11) GT+TT=110 (0.53) G=281 (0.68)
T=133 (0.32)
GG=39 (0.48)
GT=34 (0.42)
TT=8 (0.10) GT+TT=42 (0.52) G=112 (0.69)
T=50 (0.31)
1.03 (0.75-1.51) 0.89 0.97 (0.42-1.51) 0.87 0.90 (0.71-.1.42) 0.95 0.94 (0.64-1.40) 0.84
27 VNTR bb=7 (0.03)
ba=49 (0.25) aa=144 (0.72) ba+aa=193 (0.97) b=63 (0.16)
a=337 (0.84)
bb=217 (0.61)
ba*=121 (0.34)
aa*=18 (0.05) ba+aa*=139 (0.39) b=555 (0.78)
a*=157(0.22)
bb=61 (0.44)
ba*=63 (0.45) aa*=15 (0.11) ba+aa*=78 (0.56) b=185 (0.67)
a*=93 (0.33)
1.85 (1.22-2.81) 0.005 2.96 (1.41-3.22) 0.006 2.00 (1.34-2.97) 0.008 1.77 (1.31-2.41) 0.003 bb=202 (0.59)
ba*=121 (0.35) aa*=21 (0.06) ba+aa*=142 (0.41) b=523 (0.76)
a*=161 (0.24)
bb=59 (0.44)
ba*=57 (0.43) aa*=17 (0.13) ba+aa*=74 (0.56) b=175 (0.66)
a*=91 (0.34)
1.61 (1.05-2.47) 0.04 2.77 (1.37-3.59) 0.006 1.78 (1.19-2.67) 0.006 1.69 (1.24-2.30) 0.001 bb=117 (0.57)
ba*=75 (0.36) aa*=14 (0.07) ba+aa*=89 (0.41) b=310 (0.75)
a*=104 (0.25)
bb=37 (0.46)
ba*=33 (0.41) aa*=11 (0.13) ba+aa*=44 (0.54) b=107 (0.66)
a*=55 (0.34)
1.39 (1.07-2.42) 0.03 2.48 (1.84-3.92) 0.006 1.56 (1.10-2.61) 0.62 1.53 (1.13-2.27) 0.004
*P<0.05 P values for the model are adjusted for potential confounders which include age, gender, HbA1c, duration of diabetes, duration of hypertension, smoking, systolic blood pressure, triglyceride levels, ACEI/ARB, serum creatinine and eGFR
Table 3: Genotype and Allele frequencies of eNOS variants in T2DM patients (DM vs. DNP).