|
Target organism |
Primer/Probe |
Sequence (5' - 3') |
Size (bp) |
Conc. [pmol/µL] |
Reference |
| All Bacteria |
Fwd primer |
ACT CCT ACG GGA GGC AG |
468 |
10 |
[1] |
| Rev primer |
GAC TAC CAG GGT ATC TAA TCC |
10 |
| Probe |
(6-FAM)-TGC CAG CAG CCG CGG TAA TAC-(BHQ-1) |
2 |
| Clostridium cluster IV (Ruminococcaceae) |
Fwd primer |
GCA CAA GCA GTG GAG T |
239 |
4 |
[2] |
| Rev primer |
CTT CCT CCG TTT TGT CAA |
4 |
| Probe |
[3]-AGG GTT GCG CTC GTT-(BHQ-1) |
2 |
|
| Cluster XIVa (Lachnospiraceae) |
Fwd primer |
GCA GTG GGG AAT ATT GCA |
477 |
5 |
[4] |
| |
Rev primer |
CTT TGA GTT TCA TTC TTG CGA A |
5 |
|
| |
Probe |
(6-FAM)-AAA TGA CGG TAC CTG ACT AA-(BHQ-1) |
1,5 |
|
| Bacteroidetes |
Fwd primer |
GAG AGG AAG GTC CCC CAC |
106 |
3 |
[5] |
| Rev primer |
CGC TAC TTG GCT GGT TCA G |
3 |
| Probe |
(6-FAM)-CCA TTG ACC AAT ATT CCT CAC TGC TGC CT-(BHQ-1) |
1 |
| Bifidobacterium spp. |
Fwd primer |
GCG TGC TTA ACA CAT GCA AGT C |
125 |
3 |
[6] |
| Rev primer |
CAC CCG TTT CCA GGA GCT ATT |
3 |
| Probe |
(6-FAM)-TCA CGC ATT ACT CAC CCG TTC GCC-(BHQ-1) |
1.5 |
| Archaea |
Fwd primer |
ATT AGA TAC CCG GGT AGT CC |
|
4 |
[7] |
| |
Rev primer |
GCC ATG CAC CWC CTC T |
1044– 1059 |
4 |
[1] |
| |
Probe |
(6-FAM)-AGG AAT TGG CGG GGG AGC AC(BHQ-1) |
915– 934 |
4 |
