Figure 4: Bar Graph showing the comparison of Semiquantitaive TLR4 mRNA expression with: (A) wound grades on wagner scale, (B) Presence or absence of Infection and (C) Gender of DFU patients. Unpaired t test was used to check the difference between the mean values of TLR4 mRNA in male and female subjects using Graph Pad Prism. One way analysis of variance (ANOVA) test was used to check the differences between means of TLR4 mRNA expression between (i) different wound grades and (ii) infected and non infected wounds. A two tailed p value < 0.05 was considered as statistically significant. Expression of TLR4 mRNA was found to be not dependent on either wound grade or infection status of the wounds (p value>0.05). Among the diabetic wounds, males showed relatively lesser amount of TLR4 mRNA transcripts with respect to females (p value=0.02, t=2.3, mean percent ratio in males=0.97 ± 0.05, mean percent ratio in females=1.23 ± 0.08).