Figure 2: CPT1b deficiency leads to suppressed fatty acid oxidation and increased glucose oxidation without lipid accumulation in the skeletal muscle
Substrate oxidation rate and lipid contents in the skeletal muscle of mice were assessed after 22 weeks of HFD feeding. (A) Ex vivo [14C]-palmitate oxidation in EDL muscle. (B) Ex vivo [14C]-D-glucose oxidation in EDL muscle. (C) [14C]-palmitate complete oxidation in isolated mitochondria from gastrocnemius muscle. (D) [14C]- palmitate incomplete oxidation in isolated mitochondria from gastrocnemius muscle. (E) Triglyceride content in gastrocnemius muscle. (F) Non-esterified free fatty acid (NEFA) content in gastrocnemius muscle. (G) Acylcarnitine content in gastrocnemius muscle. n=5-6, *p<0.05, **p<0.01, ***p<0.001.