Figure 3: Insulin signaling is well maintained in the skeletal muscle of Cpt1b+/- mice
Tissue samples from Insulin clamp study after 22 weeks of HFD feeding were subjected to Western blot analysis for the phosphorylation of Ser 302 IRS1, Ser473 AKT, Thr 202/Tyr 204 ERK1/2 and total protein of each target. (A) Gastrocnemius muscle. (B) Heart. (C) Liver. (D) GWAT. n=4, *p<0.05, **p<0.01.