Figure 3: Changes in the fluorescence of BSA or LAB+D-ribose treated with KSE.
BSA or LAB (final concentration10 mg/mL) in the presence of D-ribose (final concentration 1 M) was kept at 37°C in Tris-HCl buffer (pH 7.4). KSE (4 and 8 μL) was mixed with samples of BSA (Figure 3-a) or LAB (Figure 3-b) +D-ribose for up to 10 days. The fluorescence intensity of glycation was recorded (λex 360 nm; λem 465 nm).BSA (or LAB) and D-ribose were used as a control. Aliquots were taken for measurements of fluorescence spectra (λex 360 nm; λem 465 nm). Values are the mean ± SD of three measurements. **p<0.01 compared with the controls.