Figure 4: Lipid contents in the gastrocnemius muscle at different time points after HFD feeding. Gastrocnemius muscle samples were used for the lipid extraction. Lipid contents were measured using (A) NEFA-HR Kit (Wako) for NEFA, (B) Triglyceride Quantification Kit (BioVision K622-100) for triglyceride. *p<0.05 WT vs. Cpt1b+/-, n=5 per group.