Figure 3: Alternative splicing of a minigene reporter construct containing the RGS9 TTTCT I/D alleles. A. The RGS9 I and D allele fragment-containing minigenes were separately co-transfected into cells with or without either PTB (left panel) or nPTB (right panel). The minigene transcripts were visualized by reverse transcription, PCR amplification and agarose gel electrophoresis. The bands “a”, “b”, and “c” represent alternatively spliced products of the minigene reporter. B. Densitometric quantification of the relative intensity of band “a”, i.e. full-length alternatively spliced product, expressed as percentage of the total intensity from “a”, “b”, and “c” with and without co-expression of PTB (left panel) or nPTB (right panel) (*p< 0.01, t test).