Figure 1: Insulin mediated phosphorylation of IRS-1 Ser307 in the triceps of C57BL/6 and S6K2-/- mice.
A, IRβ protein levels in designated tissue lysates. Starting at 7-10 weeks of age, groups (n=8) of C57BL/6 and S6K2-/- male mice were fed SD or HFD for 31 weeks.
B, IRS-1 protein levels determined by ELISA. C, IRβ phosphorylation of panTyr in response to insulin. Tissues were collected 10 min after tail vein injection of insulin (0.75 U/kg). D, phosphorylation of IRS-1 Ser307 determined by ELISA **P<0.01,***P<0.001.