Figure 3: LASY mRNA and LA levels in skeletal muscle and adipose tissue from ob/ob mice treated with compound 1
A,B: Induction of LASY mRNA levels in skeletal muscle (A) and adipose tissue (B): Real time PCR quantification was done using the SYBR green Full Velocity real time PCR kit (Stratagene) and mouse LASY primers (SABiosciences, Inc). Ct values obtained from LASY primers were corrected for Ct values from mouse Actin primers. Percent changes compared to vehicle control are shown. N=5 tissues per group. P values were calculated using the two samples, paired t test. P=0.01 for compound 1 versus vehicle for skeletal muscle and adipose tissue
C,D: Induction of LA levels in skeletal muscle (C) and adipose tissue (D): Tissue lysates were analyzed by Western blot using antibodies to LA associated with PDC and KGD. Bands were quantitated using TotalLab software (Newcastle upon Tyne, UK). LA levels relative to Actin (LA/Actin) are shown. N=5 for each group. Data shown are means ± SD. P values were calculated using two sample, paired t test. Skeletal muscle: *P=0.02 and P=0.03 for compound 1