Figure 5: Increased acellular capillaries in STZ-injected TSP1-/- male mice. Wild-type and TSP1-/- 8 week old male mice were injected with STZ and trypsin digestion of retinal wholemounts were prepared 3 months later (5 months of age). Trypsin digest preparation of retinas from STZ-induced diabetic wild-type and TSP1-/- male mice were quantified for the number of acellular capillaries (scale bar= 50 μM). Arrow heads point to acellular capillaries. Please note increased numbers of acellular capillaries were observed in STZ-injected TSP1-/- male mice compared to their wild-type STZinjected counterparts (*P < 0.05; n ≥ 5 mice).