Figure 6: Akita/+ male mice with TSP1 inducibly down-regulated demonstrated increased numbers of acellular capillaries. The generation of Akita/+; TieTSP1 mice is detailed in Panel A. In Panel B TSP1 staining of the frozen sections from eyes of 8.5 month Akita/+ and Akita/+; TieTSP1 male mice are shown (scale bar= 50μM). Mice were feed doxocycline chow from age 6 weeks through the duration of the experiment (7 months). Note decreased TSP1 expression in retinas from Akita/+; TieTSP1 male mice compared to Akita/+ male mice. Arrow heads point to acellular capillaries. Retinal trypsin digests and the quantitative assessment of the number of acellular capillaries are shown in the lower panels. Please note that more acellular capillaries formed in Akita/+; TieTSP1 male mice compared to Akita/+ male mice (*P < 0.05; n ≥ 5 mice).