NOS3
gene

Disease

 

T1DM

T2DM

Wild

Rare

Wild

Rare

 

 

4b/4a

CF

 

alleles
(%)

87.50

12.50

75.81

24.19

DPN

87.50

12.50

84.26

15.74

C

85.06

14.94

86.27

13.73

CF/DPN

 

P Value
(χ2)

1.00 (0.00)

0.17 (1.84)

CF/C

0.66 (0.20)

0.09 (2.90)

DPN/C

0.001 (10.20)

0.68 (0.17)

 

rs1799983
(G894T)

CF

 

alleles
(%)

44.23

55.77

69.35

30.65

DPN

60.00

40.00

52.88

47.12

C

55.19

44.81

61.11

38.89

CF/DPN

 

P Value
(χ2)

0.13 (2.25)

0.037 (4.36)

CF/C

0.17 (1.87)

0.28 (1.16)

DPN/C

0.59 (0.30)

0.23 (1.46)


NOS3, endothelial nitric oxide synthase; CF, free microvascular complications; DPN, diabetic neuropathy; C, combined diabetic microvascular complications (retinopathy, neuropathy, nephropathy). Significance (P Value) was 0.05 or less as accepted.  Note, in T1DM the number of  NOS3 4a carriers was significantly higher in C as compared with DPN subjects, suggesting that the rare allele was a risk factor for the prevalence of more than one microvascular disorder. In T2DM NOS3 894T allele might have an important role in the prevalence of DPN as the rare allele was significantly higher as compared with CF.

Table 1: Allele frequencies of NOS3 4b/4a  and  NOS3 rs1799983 in diabetic neuropathy