Figure 4: Myocardial apoptosis. (A) Paraffin sections were stained for apoptosis using the DeadEnd Fluorometric TUNEL assay (green, upper panel). DAPI (blue, lower panel) was used to stain for nuclei. Magnification = 40 X. (B) PARP cleavage was detected by immunoblotting using anti-PARP. Upper panel, PARP protein and cleavage product; lower panel, actin as loading control.