Figure 5: Inflammatory infiltration and fibrosis. (A) Granulocyte infiltration. Paraffin sections of heart tissues were stained for Naphthol AS-D chloroacetate esterase activity. Increased esterase-positive granulations (red and brown) shown by arrows indicate granulocyte infiltration. Magnification = 40 X. (B) Quantitative data of experiment (A). (C) Macrophage infiltration. Mac-2-positive macrophages were shown by fluorescent staining using an anti-Mac-2 antibody of paraffin sections of mouse hearts. DAPI staining was used to show the nuclei. (D) Quantification of (C). (E) Fibrosis. Paraffin heart sections were stained with picrosirius red. Increased collagen formation (red) indicates fibrosis. Magnification = 40X. (F) Quantification of (E).*, p<0.05; **, p<0.01.