Figure 2: The effect of DPP4 inhibition on fibronectin expression (a) DPP4 inhibition with linagliptin (30 nM) reduced high glucose induced fibronectin expression HK2 cells were treated with high glucose (30 mM) and linagliptin (30 nM) for 24 h. Cell lysate was collected and fibronectin was quantitated using western blot. N=4, * p<0.05 vs. ctrl, † p<0.05 vs. 30 mM Glu. (b) DPP4 inhibition with linagliptin alone did not affect basal levels of FN. TGFβ 1 is used as a positive control. N=4, *p<0.05 vs. ctrl.