Experimental details Turbidity of test Pseudomonas fluorescens SM1culture at various time intervals
Time (h) O.D550*
Pseudomonas medium alone
(-ve control)
0
24
48
0.36 ± 0.03
1.42 ± 0.35
1.60 ± 0.56
Pseudomonas broth supplemented
with 4X test heavy metals mixture
0
24
48
0.32 ± 0.04
1.43 ± 0.45
1.58 ± 0.52
Pseudomonas broth supplemented
with 4X test pesticides mixture
0
24
48
0.37 ± 0.05
1.40 ± 0.61
1.68 ± 0.48
Pseudomonas broth supplemented
with 4X test phenolics mixture
0
24
48
0.30 ± 0.04
1.41 ± 0.38
1.60 ± 0.32
Pseudomonas broth supplemented
with 4X test toxicants (heavy metals + pesticides + phenolics) in combination
0
24
48
0.28 ± 0.04
1.45 ± 0.75
1.62 ± 0.65
Pseudomonas broth prepared in filter sterilized industrial sewage water 0
24
48
0.19 ± 0.05
1.31 ± 0.67
1.54 ± 0.62
*O.D. values are given as the statistical mean of the three independent experiments carried out using experimental tubes in triplicate
Table 5: Growth characteristics of the most tolerant Pseudomonas fluorescens strain SM1 in the presence and absence of major water toxicants.