Experimental details

Turbidity of test Pseudomonas fluorescens SM1culture at various time intervals

Time (h)

O.D550*

Pseudomonas medium alone
(-ve control)

0
24
48

0.36±0.03
1.42±0.35
1.60±0.56

Pseudomonas broth supplemented
with 4X test heavy metals mixture

0
24
48

0.32±0.04
1.43±0.45
1.58±0.52

Pseudomonas broth supplemented
with 4X test pesticides mixture

0
24
48

0.37±0.05
1.40±0.61
1.68±0.48

Pseudomonas broth supplemented
with 4X test phenolics mixture

0
24
48

0.30±0.04
1.41±0.38
1.60±0.32

Pseudomonas broth supplemented
with 4X test toxicants (heavy metals + pesticides + phenolics) in combination

0
24
48

0.28±0.04
1.45±0.75
1.62±0.65

Pseudomonas broth prepared in filter sterilized industrial sewage water

0
24
48

0.19±0.05
1.31±0.67
1.54±0.62

*O.D. values are given as the statistical mean of the three independent experiments carried out using experimental tubes in triplicate
Table 5: Growth characteristics of the most tolerant Pseudomonas fluorescens strain SM1 in the presence and absence of major water toxicants.