Curing agents treatments

concentration

No. of colonies tested  from isolates

Colonies lost antibiotic resistance

Lab2

Lab11

Lab13

Lab2

Lab11

Lab13

(1) EtBr

10 µg/ml

50

50

50

0

0

0

(2) SDS

0.8 mg/ml

50

50

50

0

0

0

(3) EtBr at. 42°C

10 µg/ml

50

50

50

0

0

0

(4) SDS at 42°C

0.8 mg /ml

50

50

50

3

2

2

Table 2: Effect of different curing agents on cured plasmid conferring resistance to antibiotics in lactobacillus isolates.