Figure 5:Enzyme activity analyses on double carbon source from P. putida CSV86. Cells were grown on: (a) PA, 0.1 % + glucose, 2 %; (b) 4-HPA, 0.1 % + glucose, 2 %. Pre-inoculum was prepared on PA (a) or 4-HPA (b). Bars represents the specific activity of enzymes in nmol.min- 1.mg- 1 of protein, Zwf, 4-HPAH, 3,4-DHPADO. Growth profile on double carbon source is indicated by (●). Reducing sugar concentration in the spent medium is represented by (▼). 4-HPAH and 3,4-DHPA-DO activities were not detected at 18 and 20 h growth points on double carbon sources.