Substrate* O2 uptake rate (nmol.min-1.mg-1) by whole-cells grown on
PA 4-HPA Glucose
PA 21.4 ± 1.4 0.8 ± 0.6 tr
4-HPA 2.3 ± 0.1 12 ± 0.3 tr
3.4-DHPA 0.9 ± 0.6 14 ± 0.7 tr
2.5-DHPA 2.25 ± 1.1 tr tr
Succinate tr 3.5 ± 0.09 tr
3,4-DHBA tr tr tr
Catechol tr tr tr
* Cells grown on various carbon source (PA, 4-HPA or Glucose) are monitored for
their O2 uptake rates on these substrates (see Materials and Methods for detail).
Abbreviations: PA, Phenylacetic acid; 4-HPA, 4-hydroxyphenylacetic acid; 3,4-
DHPA, 3,4-dihydroxyphenylacetic acid; 2,5-DHPA, 2,5-dihydroxyphenylacetic acid;
3,4-DHBA, 3,4-dihydroxybenzoic acid. Rates are corrected for endogenous oxygen
uptake. Cells were grown until the late log-phase. tr, O2 uptake less than 0.5 nmol
min-1 mg-1 cells. Values after ± indicate SD observed.
Table 1: Respiration by Pseudomonas putida CSV86 cells on various aromatic intermediates.