Substrate* O2 uptake rate (nmol.min-1.mg-1) by whole-cells grown on PA 4-HPA Glucose PA 21.4 ± 1.4 0.8 ± 0.6 tr 4-HPA 2.3 ± 0.1 12 ± 0.3 tr 3.4-DHPA 0.9 ± 0.6 14 ± 0.7 tr 2.5-DHPA 2.25 ± 1.1 tr tr Succinate tr 3.5 ± 0.09 tr 3,4-DHBA tr tr tr Catechol tr tr tr * Cells grown on various carbon source (PA, 4-HPA or Glucose) are monitored for their O2 uptake rates on these substrates (see Materials and Methods for detail). Abbreviations: PA, Phenylacetic acid; 4-HPA, 4-hydroxyphenylacetic acid; 3,4- DHPA, 3,4-dihydroxyphenylacetic acid; 2,5-DHPA, 2,5-dihydroxyphenylacetic acid; 3,4-DHBA, 3,4-dihydroxybenzoic acid. Rates are corrected for endogenous oxygen uptake. Cells were grown until the late log-phase. tr, O2 uptake less than 0.5 nmol min-1 mg-1 cells. Values after ± indicate SD observed.

Table 1: Respiration by Pseudomonas putida CSV86 cells on various aromatic intermediates.