Enzymes

Specific activity (nmol.min-1.mg-1 protein) in the cell-free extract,
prepared from cells grown on

PA

4-HPA

Glucose

PA-CoA ligase

41 ± 7

nd

nd

4-HPAH

30 ± 1

627 ± 19

nd

PAH

nd

nd

nd

3,4-DHPADO

nd

745 ± 10

nd

2,5-DHPADO

nd

1.7 ± 0.3

nd

Abbreviations: PA, Phenylacetic acid; 4-HPA, 4-hydroxyphenylacetic acid; PA-CoA ligase, phenylacetyl-CoA ligase; 4-HPAH, 4-hydroxyphenylacetic acid hydroxylase; PAH, phenylacetic acid hydroxylase; 3,4-DHPADO, 3,4-dihydroxyphenylacetic acid dioxygenase; 2,5-DHPADO, 2,5-dihydroxyphenylacetic acid dioxygenase. Enzyme activities were monitored as described in ‘Materials and Methods’. nd, no activity detected. Values after ± indicate SD observed.
Table 2: Various enzymes involved in the degradation of phenylacetic acid and its derivatives in Pseudomonas putida CSV86.