Enzymes Specific activity (nmol.min-1.mg-1 protein) in the cell-free extract,
prepared from cells grown on
PA 4-HPA Glucose
PA-CoA ligase 41 ± 7 nd nd
4-HPAH 30 ± 1 627 ± 19 nd
PAH nd nd nd
3,4-DHPADO nd 745 ± 10 nd
2,5-DHPADO nd 1.7 ± 0.3 nd
Abbreviations: PA, Phenylacetic acid; 4-HPA, 4-hydroxyphenylacetic acid; PA-CoA ligase, phenylacetyl-CoA ligase; 4-HPAH, 4-hydroxyphenylacetic acid hydroxylase; PAH, phenylacetic acid hydroxylase; 3,4-DHPADO, 3,4-dihydroxyphenylacetic acid dioxygenase; 2,5-DHPADO, 2,5-dihydroxyphenylacetic acid dioxygenase. Enzyme activities were monitored as described in ‘Materials and Methods’. nd, no activity detected. Values after ± indicate SD observed.
Table 2: Various enzymes involved in the degradation of phenylacetic acid and its derivatives in Pseudomonas putida CSV86.