Nutrient amended

 Nutrient non-amended

 Sample withdrawl →

 3 day

 6 day

9 day

 12day

 3 day

 6 day

9 day

 12day

SS-HMP effluent mutagenicity with

Dose (in µl)

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9+ ve

Control

2

+

+

-

-

-

-

-

-

+

+

+

+

+

+

-

-

5

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

10

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

50

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

100

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

P.chrysosporium

2

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

+

-

+

-

+

-

-

-

-

-

+

-

-

-

-

-

10

+

-

+

-

+

-

-

-

-

-

+

-

-

-

-

-

50

+

-

+

-

+

-

-

-

+

-

+

+

-

-

-

-

100

+

-

+

-

+

-

-

-

+

-

+

+

-

-

-

-

P.citrinopileatus

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

10

+

-

+

-

+

-

-

-

-

-

-

-

-

-

-

-

50

+

+

+

-

+

-

-

-

-

+

-

-

-

-

-

-

100

+

+

+

+

+

+

-

-

-

+

-

-

-

-

-

-

P. florida

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

10

+

-

+

-

+

-

+

-

-

-

-

-

-

-

-

-

50

+

-

+

-

+

-

+

-

-

-

-

-

-

-

-

-

100

+

-

+

-

+

-

+

-

+

+

-

-

-

-

-

-

(+): Mutagenicity of sample (mutagenicity ratio >2)
(-): Non- mutagenic sample (mutagenicity ratio <2)
Table 2: Pre- and post-treatment mutagenicity of Small scale handmade paper industrial effluent in nutrient amended and nutrient non-amended conditions as detected by Salmonella typhimurium strain TA98 with (S9+ve) and without S9 mix (S9-ve).