Nutrient amended

Nutrient non-amended

Sample withdrawl

3 day

6 day

3 day

6 day

LS-HMP effluent
mutagenicity with TA 98

Dose (in µl)

S9- ve

S9+ ve

S9 - ve

S9 + ve

S9- ve

S9 + ve

S9- ve

S9 + ve

Control

2

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

10

-

+

-

+

-

-

-

-

50

-

+

-

+

-

+

-

+

100

-

+

-

+

-

+

-

+

P. chrysosporium

2

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

10

-

-

-

-

-

-

-

-

50

-

-

-

-

-

-

-

-

100

-

-

-

-

-

-

-

-

P. citrinopileatus

2

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

10

-

-

-

-

-

-

-

-

50

-

+

-

+

-

-

-

-

100

-

+

-

+

-

-

-

-

P. florida

2

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

10

-

-

-

-

-

-

-

-

50

-

-

-

-

-

-

-

-

100

-

-

-

-

-

-

-

-

(+): Mutagenicity of sample (mutagenicity ratio >2)
(-): Non- mutagenic sample (mutagenicity ratio <2)
Table 3: Pre- and post-treatment mutagenicity of Large scale handmade paper industrial effluent in nutrient amended and nutrient non-amended conditions as detected by Salmonella typhimurium strain TA98 with (S9+ve) and without S9 mix (S9-ve).