Nutrient amended

Nutrient non-amended

Sample withdrawl →

3 day

6 day

9 day

12 day

3 day

6 day

9 day

12 day

LS-HMP effluent mutagenicity with TA 100

Dose
(in µl)

S9- ve

S9+ ve

S9 - ve

S9+ ve

S9 - ve

S9 + ve

S9 - ve

S9 + ve

S9 - ve

S9 + ve

S9 - ve

S9 + ve

S9 - ve

S9 + ve

S9 - ve

S9+ ve

Control

2

-

+

-

-

-

-

-

-

+

-

-

-

-

-

-

-

5

+

+

+

+

-

+

-

-

+

+

+

-

+

+

-

+

10

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

50

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

100

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

P. chrysosporium

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

10

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

50

+

-

-

-

-

-

-

-

-

+

-

-

-

-

-

-

100

+

-

-

-

-

-

-

-

-

+

-

-

-

-

-

-

P. citrinopileatus

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

10

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

50

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

100

+

-

-

-

-

-

-

-

-

+

+

-

-

-

-

-

P. florida

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

10

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

50

+

+

-

-

-

-

-

-

+

+

-

-

-

-

-

-

100

+

+

+

+

-

+

-

+

+

+

+

-

-

-

-

-

Table 4: Pre- and post-treatment mutagenicity of Large scale handmade paper industrial effluent in nutrient amended and nutrient non-amended conditions as detected by Salmonella typhimurium strain TA100 with (S9+ve) and without S9 mix (S9-ve).