S.No Characters   0day 3rd day 6th day 9th day 12th day 15th day 18th day 21th day 24th day 27th day 30th day
1 pH C 8.2  ±0.7 8.2  ± 1.2 8.2  ± 1.2 8.2  ± 0.7 8.2  ± 1.7 8.2  ± 0.2 8.2 ± 0.2 8.3  ± 0.2 8.3  ± 0.3 8.3  ± 1.2 8.3  ± 1.2
    CP 8.2  ± 1.2 8.3  ±1.2 8.3  ± 0.9 8.2  ± 0.7 8.2  ± 1.2 8.3  ± 0.6 8.2 ± 0.5 8.3 ± 0.6 8.2 ± 0.2 8.2 ± 0.2 8.3 ± 0.2
    FC1 8.2 ± 1.2 8.2 ± 1.7 8.2 ± 0.7 8.5 ± 0.5 8.6 ± 1.5 8.6 ± 1.2 8.6 ± 0.6 8.7 ± 0.5 8.8 ± 0.3 8.9 ± 0.4 8.9 ± 0.3
    FC2 8.2 ± 1.5 8.2 ± 1.6 8.2 ± 0.6 8.5 ± 0.6 8.6 ± 1.3 8.6 ± 1.3 8.6 ± 0.4 8.8 ± 0.5 8.8 ± 0.4 8.9 ± 0.3 8.9 ± 0.2
    PUF1 8.2 ± 0.8 8.2 ± 1.5 8.3 ± 0.7 8.2 ± 0.7 8.7 ± 1.4 8.7 ± 1.4 8.7 ± 0.5 8.9 ± 0.7 8.9 ± 0.3 8.9 ± 0.2 8.9 ± 0.5
    PUF2 8.2 ± 0.6 8.2 ± 1.6 8.3 ± 0.8 8.7 ± 0.6 8.7 ± 1.6 8.7 ± 1.5 8.7 ± 0.6 8.9 ± 0.6 8.9 ± 0.2 8.9 ± 0.3 8.9 ± 0.4
2 Carbonite C 60 ±1.6 60  ± 1.4 60  ± 1.5 60  ±  0.6 60 ± 0.8 60 ± 0.6 59 ± 0.6 59 ± 0.7 60 ± 0.7 59 ± 0.6 59 ± 0.9
    CP 60 ±1.3 60  ± 1.4 60  ± 1.5 60  ±  0.6 60 ± 0.8 60 ± 0.6 59 ± 0.8 59 ± 0.5 60 ± 0.4 59 ± 0.6 59 ± 0.6
    FC1 60 ± 1.5 50 ± 0.6 45  ±  0.6 40 ± 0.5 30 ± 0.6 20 ± 0.2 10 ± 0.5 5 ±  0.7 NIL NIL NIL
    FC2 60 ± 0.5 55 ± 0.5 50  ±  0.7 45 ± 1.5 35 ± 0.5 30 ± 0.5 20 ±  0.6 10 ±  0.6 NIL NIL NIL
    PUF1 60 ± 0.5 55 ± 0.5 45 ±  0.6 40 ± 1.4 30 ± 0.6 20 ± 0.6 10 ±  0.5 NIL NIL NIL NIL
    PUF2  60 ± 0.6 55 ± 0.6 50 ± 0.6 45 ± 0.9 35 ± 0.7 35 ± 0.3 10 ±  0.6 5 ±  0.6 NIL NIL NIL
3 Bicorbonate C 160 ± 1.6 160 ±  1.2 160 ±  1.8 160 ±  1.7 155 ±  1.6 155 ±  1.5 155 ±  1.7 155 ±  1.6 150 ± 1.5 140 ± 1.8 140 ± 1.6
    CP 160 ± 1.4 160 ±  1.3 160 ±  1.7 160 ±  1.6 155 ±  1.5 155 ±  1.5 155 ±  1.7 155 ±  1.3 150 ± 1.7 140 ± 1.4 140 ± 1.5
    FC1 160 ± 1.5 160 ± 1.3 140 ± 1.6 120 ± 1.5 100 ± 1.8 90  ± 1.6 70 ± 1.3 40 ± 1.5 20 ± 1.8 10 ± 1.5  5 ± 1.8
    FC2 160 ± 1.3 160 ± 1.5 160 ± 1.5 140 ± 1.8 120 ± 1.6 110 ± 1.4 90 ± 1.5 70 ± 1.8 50 ± 1.6 30 ± 1.4 10 ± 1.5
    PUF1 160 ± 1.6 150 ± 1.2 130 ± 1.3 110 ± 1.7 90 ±  1.4 80 ± 1.8 50 ± 1.6 20 ± 1.2 10 ± 1.5 5 ± 1.5 NIL
    PUF2 160 ± 1.7 160 ± 1.3 150 ± 1.7 130 ± 1.5 110 ± 1.7 90 ± 1.6 70 ± 1.4 50 ± 1.5 30 ± 1.4 10 ± 1.7 5 ± 1.5
4 BOD C 280 ± 1.8 280 ± 1.1 280  ± 1.8 280  ± 1.7 270  ± 1.6 260  ± 1.7 260  ± 1.5 260  ± 1.7 260  ± 1.4 250  ± 1.6 250  ± 1.5
    CP 280 ± 1.5 280 ± 1.2 280  ± 1.6 280  ± 1.4 270  ± 1.2 260  ± 1.3 260  ± 1.3 260  ± 1.4 260  ± 1.5 250  ± 1.7 250  ± 1.6
    FC1 280 ± 1.6 280 ± 1.3 250 ± 1.5 230 ± 1.5 200 ± 1.7 180 ± 1.6 150 ± 1.4 130 ± 1.8 110 ± 1.2 80 ± 1.3  40  ±  1.4
    FC2 280 ± 1.7 240 ± 1.1 230 ± 1.3 210 ± 1.4 200 ± 1.5 180 ± 1.8 150 ± 1.5 130 ± 1.4 110 ± 1.3 80 ± 1.7 50 ± 1.6
    PUF 1 PUF2 280 ± 1.4 280 ± 1.7 260 ± 1.1 230 ± 1.3 240 ± 1.4 220 ±  1.6 220 ± 1.5 200 ± 1.4 180 ± 1.4 180 ± 1.4 160 ± 1.4 160 ± 1.7 120 ±  1.6 120 ±  1.6 120 ± 1.6 100 ± 1.5 90 ± 1.6 70 ± 1.7 40 ± 1.4 40 ± 1.4 30 ± 1.7 20 ± 1.5
    C 496 ± 0.8 496 ±  0.7 496 ± 0.4 492 ± 0.2 492 ± 0.6 489 ±  0.4 489 ± 0.5 489 ±  0.6 483 ±  0.7 483 ±  0.6 483 ±  0.2
    CP 496 ±  0.6 496 ±  0.4 496 ±  0.7 492 ±  0.8 492 ±  0.5 489 ±  0.4 489 ± 0.5 489 ± 0.4 483 ±  0.8 483 ±  0.5 482 ±  0.3
    FC1 496 ± 0.8 480  ± 0.5 448 ± 0.6 412 ± 0.8 376 ± 0.4 310 ± 0.8 230 ± 0.7 182 ± 0.7 104 ± 0.6 86 ± 0.7 56 ± 0.5
    FC2 496 ±  0.6 490 ± 0.2 468 ± 0.6 433 ± 0.5 386 ± 0.7 330 ± 0.3 270 ± 0.2 203 ± 0.3 126 ± 0.5 102 ± 0.5 82 ± 0.4
    PUF 1 496 ± 0.5 464 ± 0.6 426 ± 0.4 384 ± 0.7 314 ± 0.6 278 ± 0.4 198 ± 0.6 144 ± 0.6 96 ± 0.3 68 ± 0.6 24 ± 0.3
    PUF2 496 ±  0.6 480 ± 0.5 446 ± 0.4 397 ± 0.6 328 ± 0.7 296 ± 0.6 225 ± 0.3 172 ± 0.5 112 ± 0.6 68 ± 0.6 49 ± 0.5
6 DO C 1.67 ±  0.8 1.67 ± 0.1 1.67 ±  0.8 1.67 ±  0.6 1.67 ± 0.3 1.67 ± 0.4 1.82 ±  0.3 2.2 ±  0.3 2.2 ±  0.7 2.2 ± 0.5 2.2 ± 0.3
    CP 1.67 ±  0.6 1.67 ± 0.2 1.67  ±  0.1 1.67 ±  0.5 1.67 ± 0.4 1.67 ± 0.2 1.82 ±  0.5 2.2 ±  0.2 2.2 ±  0.1 2.2 ± 0.4 2.2 ± 0.4
    FC1 1.67 ±  0.5 1.67 ± 0.3 2.33± 0.6 2.79 ± 0.5 4.56 ± 0.7 5.9 ± 0.3 6.8 ± 0.2 7.42 ± 0.3 7.2 ± 0.6 7.4 ± 0.5  8 ±  0.5
    FC2 1.67 ±  0.4 1.67 ±0.2 2.79 ± 0.5 3.35 ± 0.4 4.67 ± 0.5 5.7 ± 0.6 6.9 ± 0.5 7.2 ± 0.2 7.8 ± 0.5  8 ± 0.5 8.6 ± 0.5
    PUF  1 1.67±  0.8 2.58 ± 0.1 2.79 ± 0.6 3.35 ± 0.4 5.6 ± 0.4 6.37 ± 0.5 7.21 ± 0.1 7.82 ± 0.1 8.2 ± 0.3 8.7 ± 0.6 9.2 ± 0.4
    PUF 2 1.67 ±  0.7 2.78 ± 0.2 3.35 ± 0.8 4.56 ± 0.6 5.8 ± 0.5 6.4 ± 0.4 7.8 ± 0.3 8.4 ± 0.4 8.9 ± 0.2 9.2 ± 0.4 9.8 ± 0.6
7 Nitrate C 168  ± 1.6 158  ± 2.1 158  ± 2.1 157  ± 1.9 156  ± 2.1 156  ± 2.0 155  ± 2.1 155  ± 2.0 154  ± 1.9 154  ± 2.2 154  ± 2.1
    CP 168  ± 1.6 158  ± 2.0 158  ± 2.1 157  ± 1.9 156  ± 2.1 156  ± 2.0 155  ± 2.1 155  ± 2.0 154  ± 1.9 154  ± 2.2 154  ± 2.1
    FC1 168  ± 1.4 158  ± 2.1 156 ± 1.9 140 ± 1.8 135 ± 1.9 127 ± 2.1 122 ± 2.0 115 ± 1.9 110 ± 2.0 92 ± 1.9 80 ± 2.0
    FC2 168  ± 1.5 158  ± 1.9 156 ± 2.1 145 ± 1.7 138 ± 2.0 130 ± 2.0 128 ± 2.0 119 ± 1.9 112 ± 1.9 99 ± 2.0 84 ± 2.1
    PUF 1 168  ± 1.7 158  ± 2.0 154 ± 2.0 160 ± 1.7 122 ± 1.9 110 ± 2.1 108 ± 2.1 102 ± 2.0 90 ± 2.0 78 ± 2.1 60 ± 2.0
    PUF 2 168  ± 1.6 158  ± 2.1 155 ± 2.1 132 ± 1.9 125 ± 1.9 117 ± 2.1 112 ± 2.0 108 ± 2.1 101 ± 2.1 82 ± 2.0 64 ± 2.1
8 Nitrate C 74 ± 1.5 74 ± 1.8 70 ± 1.5 73 ± 1.4 72 ± 1.4 71 ± 1.5 71 ± 1.6 71 ± 1.5 70 ± 1.4 69 ± 1.8 69 ± 1.4
    CP 74 ± 1.4 74 ± 1.6 73 ± 1.6 73 ± 1.7 72 ± 1.3 71 ± 1.2 71 ± 1.7 71 ± 1.1 70 ± 1.3 69 ± 1.9 69 ± 1.2
    FC1 74 ± 1.1 74 ± 1.7 70 ± 1.5 63 ± 1.5 56 ± 1.4 40 ± 1.3 31 ± 1.4 22 ± 1.4 70 ± 1.3 4  ± 1.3 NIL
    FC2 74 ± 1.4 74 ± 1.5 70 ± 1.4 62 ± 1.4 51 ± 1.5 40 ± 1.4 36 ± 1.3 24 ± 1.5 12 ± 1.5 12  ± 1.5 NIL
    PUF 1 74 ± 1.2 71 ± 1.7 68 ± 1.4 54 ± 1.6 48 ± 1.6 36 ± 1.5 24 ± 1.5 15 ± 1.6 7  ± 1.6 NIL NIL
    PUF 2 74 ± 1.3 73 ± 1.8 64 ± 1.5 53 ± 1.5 36 ± 1.6 22 ± 1.3 17 ± 1.6 10 ± 1.4 4  ± 1.4 NIL NIL
9 Ammonia C 78 ±  1.8 78 ±  1.0 77 ±  0.6 77 ± 0.7 76 ±  0.9 76 ±  0.8 74 ±  0.4 74 ±  0.7 72 ± 0.3 72 ± 0.6 72 ± 0.5
    CP 78 ±  1.7 78 ±  1.2 77 ±  0.5 77 ±  0.6 76 ±  0.8 76 ±  0.5 74 ±  0.8 74 ±  0.6 72 ± 0.7 72 ± 0.3 72 ± 0.4
    FC1 78 ±  1.6 76 ±  1.5 65 ± 1.1 52 ± 0.6 41 ± 0.8 30 ± 0.6 23 ± 0.7 17 ± 0.3 5 ± 0.9 NIL NIL
    FC2 78 ±  1.3 76 ±  1.7 72 ± 1.0 62 ± 0.4 50 ± 0.6 42 ± 0.7 36 ± 0.2 20 ± 0.4 10 ± 0.3 5 ± 0.6 NIL
    PUF 1 78 ±  1.7 71 ±  1.7 58 ± 1.4 46 ± 0.2 30 ± 0.4 24 ± 0.6 12 ± 0.7 8 ± 0.3 NIL NIL NIL
    PUF 2 78 ±  1.2 75 ±  1.3 66 ± 1.3 58 ± 0.5 44 ± 0.5 32 ± 0.5 24 ± 0.6 14 ± 0.8 5 ± 0.5 NIL NIL
10 Total phosphours C 48 ± 0.1 48 ± 0.3 47 ± 0.6 46 ± 0.5 46 ± 0.1 45 ± 0.5 44 ± 0.3 44 ± 0.7 42  ±  0.5 41  ±  0.8 41  ±  0.4
    CP 48 ± 0.2 48 ± 0.4 47 ± 0.5 46 ± 0.4 46 ± 0.2 45 ± 0.3 44 ± 0.5 44  ±  0.6 42  ±  0.4 41  ±  0.6 41  ±  0.7
    FC1 48 ± 0.8 48 ± 0.3 46 ± 0.3 42 ± 0.5 36 ± 0.5 28 ± 0.1 24 ± 0.4 20  ±  0.8 20  ±  0.9  8  ±  0.5  8  ±  0.6
    FC2 48 ± 0.6 48 ± 0.2 46 ± 0.8 40 ± 0.8 32 ± 0.7 24 ± 0.5 22 ± 0.5 17  ±  0.6 17  ±  0.8  5  ±  0.4  5  ±  0.5
    PUF1 48 ± 0.4 48 ± 0.1 44 ± 0.4 40 ± 0.7 30 ± 0.5 20 ± 0.4 12 ± 0.3 8 ± 0.6 8  ±  0.1 NIL NIL
    PUF2 48 ± 0.5 46 ± 0.6 42 ± 0.7 38 ± 0.4 28 ± 0.7 18 ± 0.8 10 ± 0.5 5  ±  0.7 5  ±  0.2 NIL NIL
11 Organic phosphate C 30  ±  1.7 30  ± 0.9 29  ±  0.8 29 ± 0.3 29  ±  0.8 28  ± 0.5 28  ± 0.3 28  ± 0.9 26  ± 0.4 26  ± 0.2 26  ± 0.8
    CP 30  ±  1.8 30  ±  0.4 29 ± 0.6 29  ± 0.5 29  ±  0.7 28  ± 0.7 28  ± 0.4 28  ± 0.7 26  ± 0.6 26  ± 0.3 NIL
    FC1 30  ±  1.6 30  ±  0.5 30 ± 0.7 25 ± 0.5 22  ±  0.5 18  ± 0.5 16  ± 0.7  9  ± 0.2  7 ± 0.5  3  ± 0.5 NIL
    FC2 30  ±  1.4 30  ±  0.8 30 ± 0.6 26 ± 0.8 18  ±  0.6 12  ± 0.3 12  ± 0.5  5  ± 0.5  4  ± 0.6  2  ± 0.4 NIL
    PUF1 30  ±  1.4 30  ±  0.7 28 ± 0.7 26 ± 0.4 20  ± 0.7 12  ± 0.4  7  ± 0.6  5  ± 0.4 NIL NIL NIL
    PUF2 30  ±  1.7 28  ±  0.6 26 ± 0.4 26  ± 0.6 18  ± 0.4 10  ± 0.8  5  ± 0.4  3  ± 0.6 NIL NIL NIL
12 Inorganic  phosphate C 18  ± 0.1 18  ± 0.2 18  ± 0.8 17  ± 0.1 17  ± 0.9 17  ± 0.8 16  ± 0.3 16  ± 0.4 16  ± 0.3 15  ± 0.8 15  ± 0.4
    CP 18  ± 0.3 18  ± 0.3 18  ± 0.6 17  ± 0.4 17  ± 0.3 17  ± 0.6 16  ± 0.4 16  ± 0.5 16  ± 0.1 15  ± 0.7 15  ± 0.3
    FC1 18  ± 0.2 18  ± 0.1 16  ± 0.4 16  ± 0.6 14  ± 0.5 12  ± 0.2 10 ± 0.6  8  ± 0.7  4  ± 0.5   3 ± 0.4 NIL
    FC2 18  ± 0.3 18  ± 0.2 18  ± 0.9 16  ± 0.4 14  ± 0.7 10  ± 0.3  8  ± 0.8   5 ± 0.3  3  ± 0.6  2  ± 0.5 NIL
    PUF1 18  ± 0.4 18  ± 0.1 16  ± 0.6 14  ± 0.3 10  ± 0.6   8  ± 0.5  5 ± 0.4   3 ± 0.8 NIL NIL NIL
    PUF2 18  ± 0.3 18  ± 0.3 16  ± 0.5 14  ± 0.2 16  ± 0.4   8 ± 0.7  5 ± 0.3   2 ± 0.4 NIL NIL NIL
13 Calcium C 67.28  ± 1.4 67.28  ± 1.4 67.28 ± 1.2 62.25 ± 1.3 64.31 ± 1.5 64.31 ± 1.2 64.31 ± 1.5 62.3 ± 1.3 60.09 ± 1.4 60.09 ± 1.5 60.09 ± 1.4
    CP 67.28  ± 1.6 67.28  ± 1.3 67.28 ± 1.3 62.25 ± 1.4 64.31 ± 1.2 64.31 ± 1.3 64.31 ± 1.2 62.3 ± 1.4 60.09 ± 1.3 60.09 ± 1.2  60.09 ± 1.3
    FC1 67.28  ± 1.3 54.34  ± 1.2 54.12 ± 1.3 46.52 ± 1.2 37.84 ± 1.4 29.43 ± 1.5 23.36 ± 1.3 19.45 ± 1.5 16.82 ± 1.2 10.61 ± 1.3 6.2 ± 1.3
    FC2 67.28  ± 1.7 63.07 ± 1.5 63.07 ± 1.1 58.46 ± 1.1 48.05 ± 1.3 40.07 ± 1.1 33.64 ± 1.1 26.12 ± 1.1 16.44 ± 1.3  9.41 ± 1.2 4.12 ± 1.2
    PUF1 67.28  ± 1.5 51.24 ± 1.2 48.23 ± 1.5 40.42 ± 1.3 33.64 ± 1.2 25.23 ± 1.3 20.17 ± 1.2 12.61 ± 1.2  9.42 ± 1.5  4.2 ± 1.5 NIL
    PUF2 67.28  ± 1.2 52.49 ± 1.4 51.66 ± 1.5 49.25 ± 0.9 39.64 ± 1.3 27.23 ± 1.5 20.12 ± 1.5 16.82 ± 1.3 12.61 ± 1.2  8.41 ± 1.3 NIL
14 Magnesium C 54.34 ± 2.1 54.34 ± 1.1 54.34 ± 1.9 52.26  ± 1.6 51.08  ± 1.7 51.08  ± 1.5 51.08  ± 1.7 50.02  ± 1.3 49.84  ± 1.6 48.67  ± 1.7 48.67  ± 1.7
    CP 54.34 ± 2.2 54.34 ± 1.3 54.34 ± 1.8 52.26  ± 1.5 51.08  ± 1.6 51.08  ± 1.4 51.08  ± 1.6 50.02  ± 1.4 49.84  ± 1.5 48.67  ± 1.6 48.67  ±  1.6
    FC1 54.34 ± 2.0 54.34 ± 1.2 50.45 ± 1.6 46.24  ± 1.3 39.56  ± 1.5 33.27  ± 1.7 29.56  ± 1.4 23.12  ± 1.8 19.41  ± 1.6 10.56  ± 1.8  5.41  ± 1.8
    FC2 54.34 ± 1.8 52.49 ± 1.5 50.61 ± 1.9 45.64  ± 1.7 41.05  ± 1.6 35.64  ± 1.6 29.23  ± 1.8 23.12  ± 1.7 16.13  ± 1.5 8.35  ± 1.3  4.12  ± 1.6
    PUF1 54.34 ± 2.4 54.34 ± 1.1 50.61  ± 1.7 42.39  ± 1.8 33.27  ± 1.7 26.12  ± 1.5 23.41  ± 1.7 19.41  ± 1.5 14.52  ± 1.6 6.53  ± 1.5 NIL
    PUF2 54.34 ± 1.9 52.24 ± 1.6 50.23  ± 1.4 46.13  ± 1.3 35.23  ± 1.5 29.21  ± 1.6 25.23  ± 1.4 20.32  ± 1.4 16.41  ± 1.3  8.32  ± 1.5 NIL
15 Chloride C 4699 ± 1.9 4699 ± 1.7 4690 ± 1.1 4690 ± 1.3 4690 ± 1.6 4688 ± 1.9 4685 ± 1.2 4626  ± 1.7 4626  ± 1.2 4628  ± 1.7 4624  ± 1.6
    CP 4699 ± 1.8 4699 ± 1.6 4690 ± 1.2 4690 ± 1.4 4690 ± 1.5 4688 ± 1.7 4685  ± 1.7 4626  ± 1.6 4626  ± 1.3 4628  ± 1.9 4624  ± 1.5
    FC1 4699 ± 1.6 4408 ± 1.6 4166 ± 1.2 3892 ± 1.2 3476 ± 1.5 3224 ± 1.8 3198 ± 1.5 3092  ± 1.5 2622  ± 1.5 2886  ± 1.8 2785  ± 1.4
    FC2 4699 ± 1.5 4498 ± 1.8 4244 ± 1.3 3928 ± 1.2 3746 ± 1.7 3426 ± 1.6 3244  ± 1.6 3122  ± 1.6 2966  ± 1.6 2894  ± 1.6 2812  ± 1.6
    PUF1 4699 ± 1.8 4378 ± 1.6 4016 ± 1.2 3892 ± 1.8 3448 ± 1.5 3206 ± 1.5 2989  ± 1.5 2848  ± 1.8 2512  ± 1.6 2392  ± 1.8 2346  ± 1.7
    PUF2 4699 ± 1.7 4522 ± 1.8 4112 ± 1.3 3792 ± 1.7 3524 ± 1.8 3298 ± 1.8 3092  ± 1.8 2966  ± 1.7 2786  ± 1.4 2942  ± 1.7 2384  ± 1.8
C - Control; CP- Effluent inoculated with PUF cubes (without organism), FC1 - Effluent treated with free cells of O. brevis; FC2 - Effluent treated with free cells of W. prolifica; PUF1 - Effluent treated with PUF immobilized O. brevis; PUF2 - Effluent treated with PUF immobilized W. prolific
Table 2: Physico-chemical characteristics of cyanobacteria inoculated and uninoculated (control).