Factors

Level 1

Level 2

Level 3

Level 4

pH

5

6

7

8

Temperature (°C)

25

30

35

40

Dye concentration (ppm)

25

50

75

100

Carbon source

Glucose

Starch

Sucrose

Lactose

Nitrogen source

Yeast extract

Peptone

(NH4)NO3

(NH4)2(SO44)

Table 1: Different parameters and levels used, to design orthogonal array.