Figure 1: Yeast lysate mediated demeton-S methyl detoxification measured by the reduced ability of the agent to inhibit AChE activity. Demeton-S methyl (50 μM) hydrolysis assays were performed with lysates (10 μg) prepared from control (OPH negative), wild-type OPH, or S308L-OPH yeast. After varying incubation periods at 25°C, the ability of demeton-S methyl to inhibit AChE was measured. The S308L-OPH mediated reduction in AChE activity is directly indicative of the reduced toxicity of demeton-S methyl. Readings represent the mean ± SD of three independent lysates.