Species/strain Genotype Source Features
E. coli
ER2738
F’proA+B+lacIqD(lacZ)M15zzf\Tn10(TetR)/fhuA2glnVD(lac-proAB)thi-1D(hsdS-mcrB)5 New England Biolabs General lab cloning strain
E. coliBL21(DE3)pLysS BF- dcm ompT hsdS(rB- mB-) gall (DE3) [pLysS Camr] Novagen Protein expression
S. cerevisiae BY4741 MATa his3D1 leu2D0 met15D0 ura3D0 Guild stock collection Wild-type strain
S. cerevisiae BY4741.OPH MATa his3D1 leu2D0 met15D0 ura3D0 OPH NEOr This study Integrated OPH
S. cerevisiae BY4741.S308L MATa his3D1 leu2D0 met15D0 ura3D0 S308L NEOr This study Integrated S308L
Table 1: Strains used and constructed in the study.