| Plasmid name |
Origin/Ref |
Features |
| pBluescript-SKII- |
Stratagene |
General cloning vector |
| pESC-URA |
Stratagene |
S. cerevisiae expression vector |
| pEGFP-N1 |
Clontech |
Mammalian reporter vector |
| pOPH-SK- |
BioS&T |
pBluescript with yeast codon optimized OPH |
| pS308L-OPH-SK- |
BioS&T |
pBluescript with yeast codon optimized variant S308L-OPH |
| pGAPDH-OPH-SK- |
This study |
GAPDH promoter 5’ of OPH |
| pGAPDH-S308L-OPH-SK- |
This study |
GAPDH promoter 5’ of S308L-OPH |
| pNEO-GAPDH-OPH-SK- |
This study |
With the NEOr gene |
| pNEO-GAPDH-S308L-OPH-SK- |
This study |
With the NEOr gene |
| pR4-NEO-GAPDH-OPH-R6-SK- |
This study |
Ribosomal sequence flanking the OPH cassette |
| pR4-NEO-GAPDH-S308L-OPH-R6-SK- |
This study |
Ribosomal sequence flanking the S308L-OPH cassette |
| pR4-NEO-GAPDH-OPH-T-R6-SK- |
This study |
Transcriptional terminator TcycI 3’ of OPH |
| pR4-NEO-GAPDH-S308L-OPH-T-R6-SK- |
This study |
Transcriptional terminator TcycI 3’ of S308L-OPH |
| pS308L-ET30a |
[6] |
E. coli S308L protein expression plasmid |
