Lysate Specific activityab Fold-difference
E. colib 0.90 (0.02) n/a
Yeastc 6.08 (0.19) 6.7
a mean μmoles hydrolyzed/min/mg total protein of three independent lysates (standard deviation)
b E. coli BL21(DE3)pLysS harboring pS308L-ET-30a
c S. cerevisiae BY4741.S308L
Table 4: Comparison of yeast and E. coli lysates harboring S308L to hydrolyze paraoxon.