OPH |
Specific activityab |
Fold-difference compared to wild-type OPH |
Demeton-S methyl |
Malathion |
Demeton-S methyl |
Malathion |
Wild-type OPH |
1.1x10-4 (0.68x10-4) |
1.0x10-4 (0.49x10-4) |
N/A |
N/A |
S308L.OPH |
116.8x10-4 (0.50x10-4) |
37.1x10-4 (0.10x10-4) |
106.2 |
37.1 |
a μmoles hydrolyzed/min/mg total protein
b average of three independent lysates (standard deviation)
N/A, not applicable |