Time SFT ± SD1 CMD-1 ± SD2 CMD-2 ± SD CMD-3 ± SD
(h) (mNm-1) (mN m-1) (mN m-1) (mN m-1)
NC3 71.34 ± 0.02 71.33 ± 0.02 71.36 ± 0.02 71.36 ± 0.02
0 43.6 ± 0.45 48.27 ± 0.025 68.33 ± 0.015 69.58 ± 0.06
24 40.3 ± 0.36 44.78 ± 0.09 57.24 ± 0.04 69.12 ± 0.01
48 34.6 ± 0.06 34.65 ±  0.11 36.72 ± 0.01 45.43 ± 0.06
72 31 ± 0.02 31.36 ± 0.023 31.28 ± 0.05 38.54 ± 0.07
96 29.9 ± 0.03 30.68 ± 0.05 30.02 ± 0.02 32.62 ± 0.055
120 31.2 ± 0.06 31.56 ± 0.026 31.98 ± 0.23 32.54 ± 0.03
1Average surface tension ± standard deviation (mN m-1) of triplicate experiments.
2Average critical micelle dilution (CMD)± standard deviation (mN m-1) of triplicate
experiments. CMD-1=10x dilution; CMD-2=100x dilution; CMD-3=1000x dilution.
3Negative control=Un-inoculated mineral salts medium (MSM)
Table 3: Relationship between surface tension and critical micelle dilution (CMD)
of the cell free broth of P. putida SOL-10 strain grown in MSM under optimized
culture conditions.