Test Measured parameter Maximum microbial concentration [CFU/mL] Suitability for compounds
Poorly soluble Volatile Adsorbing
DOC Die-Away (301 A) DOC (2 ÷ 10) • 105 - - +/-
CO2 evolution (301 B) CO2 evolution (respirometry) (2 ÷ 10) • 105 + - +
MITI (I) (301 C) O2 (respirometry) (2 ÷ 10) • 105 + +/- +
Closed bottle (301 D) O2 (respirometry) (0,5 ÷ 2.5) • 103 - - +/-
Modified OECD Screening (301 E) DOC (0,5 ÷ 2.5) • 102 +/- + +
Manometric respirometry (301 F) O2 (respirometry) (2 ÷ 10) • 105 + +/- +
+ suitable; - not suitable; +/- suitable under specific conditions
Table 3: Applicability of OECD (301 series) test methods (adapted from Pedrazzani [40]).