Test Measured parameter Maximum microbial concentration [CFU/mL] Suitability for compounds Poorly soluble Volatile Adsorbing DOC Die-Away (301 A) DOC (2 ÷ 10) • 105 - - +/- CO2 evolution (301 B) CO2 evolution (respirometry) (2 ÷ 10) • 105 + - + MITI (I) (301 C) O2 (respirometry) (2 ÷ 10) • 105 + +/- + Closed bottle (301 D) O2 (respirometry) (0,5 ÷ 2.5) • 103 - - +/- Modified OECD Screening (301 E) DOC (0,5 ÷ 2.5) • 102 +/- + + Manometric respirometry (301 F) O2 (respirometry) (2 ÷ 10) • 105 + +/- + + suitable; - not suitable; +/- suitable under specific conditions

Table 3: Applicability of OECD (301 series) test methods (adapted from Pedrazzani [40]).