Figure 4: Gas chromatographic traces of n-hexane extract of recovered fresh SAE 20w 50 engine oil from culture fluids of Pseudomonas sp. LP5 at Day 0 (A), Day 12 (B) and Day 21 (C) of incubation at room temperature. The oil components were separated on 30 m long HP-5 column (internal diameter, 0.25 mm; film thickness, 0.25 μm) in a Hewlett Packard 5890 Series II gas chromatograph equipped with flame ionization detector (FID).