Run pH Temperature (°C) Agitation (rpm) Incubation (h) Dye (mg/L) Glucose (%) Peptone (%)
1 3 20 0 0 10 0.1 0.1
2 11 20 300 72 1000 0.1 0.1
3 11 60 0 0 10 1.0 0.1
4 11 20 300 72 10 1.0 0.5
5 3 20 0 72 10 1.0 0.5
6 11 20 0 0 1000 0.1 0.5
7 3 60 300 0 1000 1.0 0.5
8 11 60 0 72 1000 1.0 0.1
9 3 20 300 0 1000 1.0 0.1
10 11 60 300 0 10 0.1 0.5
11 3 60 0 72 1000 0.1 0.5
12 3 60 300 72 10 0.1 0.1
Table 1: Plackett-Burman design for screening bioprocess variables (biotic/biotic) optimum for decolorization of Methyl Red.