Compound Sourcea Formula MWb RTc (min) Ions used ford integration Control Area (%) B207 Area (%) L201 Area (%)
Butyl acetate   C6H12O2 116 5.801 43,56,73   3.38 2.9
Isobutyric acid   C4H8O2 88 7.987 73,75,145 0.93    
Isovaleric acid   C5H10O2 102 9.845 75,73,159 0.63    
Lactic acid   C3H6O3 90 15.885 73, 147, 117 23.54    
Glycolic acid   C2H4O3 76 16.476 73,147,66 1.48    
2-Furoic acid   C5H4O3 112 18.945 125,169,95   2.87 2.87
Oxalic acid   C2H2O4 90 19.367 73,147,45 1.52 12.14 8.57
2-Hydroxy-3-methylbutyric acid   C5H10O3 118 20.662 73,145,147 2.64   0.7
Propanedioic acid   C3H4O4 104 22.548 147,73,75   4.61 4.13
3-Hydroxyisovaleric acid   C5H10O3 118 22.644 131,73,147   1.13 1.04
Benzoic acid L C7H6O2 122 23.907 105,179,77 0.31 3.68 3.23
2-hydroxy-4-methylpentanoic acid   C6H12O3 132 24.001 73,159,103 0.7   1.72
Succinic Acid   C4H6O4 118 27.344 147,73,75 47.89 1.09 0.84
2,3-dihydroxy-2-methyl-propanoic acid   C4H8O4 120 27.888 73,219,147 1.17 2.84 3.62
Fumaric acid   C4H4O4 116 28.783 245,73,147 1.44    
Citraconic acid   C5H6O4 130 28.998 147,73,45     2.02
Glutaric acid   C5H8O4 132 31.274 73,147,75 0.54 12.16 9.57
4-Hydroxyvaleric acid   C5H10O3 118 32.971 73,117,147 0.76   2.76
Malic acid   C4H6O5 134 35.106 73,147,233 5.53    
trans-3-Hexenedioic acid   C6H8O4 144 35.343 73,147,75   2.63 2.54
Pyroglutamic acid   C5H7NO3 129 35.983 156,73,147 10.42    
2-Hydroxyphenylacetic acid L,H C8H8O3 152 37.68 73,147,253   6.94 8.18
Pimelic acid   C7H12O4 160 39.096 73,75,55   1.27 1.23
4-hydroxybenzoic acid L,H C7H6O3 138 39.798 73,267,193 0.68 6.31 4.71
4-hydroxyphenylacetic acid L,H C8H8O3 152 40.335 73,179,75 0.42 10.91 8.43
Suberic acid   C8H14O4 174 42.562 73,75,303   3.4 3.92
Vanillic acid L,G C8H8O4 168 44.874 297,73,312 2.04 9.59 8.36
Azelaic acid   C9H14O4 188 45.991 73,75,55 0.51 3.34 2.54
2-Hydroxy-3-(4-hydroxyphenyl) propanoic acid L,H C9H10O4 182 49.687 73,179,147   1.13 1.64
Ferulic acid L,G C10H12O4 196 52.983 209,73,340   1.92 1.88
Total lignin content (%) 1.31 11.37 9.92
a L, Lignin; G, guaiacyl type; H, hydroxyphenyl type b Molecular weight c Retention times of TMS derivatives d Ions of TMS derivative
Table 5: Results obtained from GC-MS analysis of the derivatized extracts from untreated softwood control and softwood samples treated by B207 and L201, respectively.